12/29/2023 0 Comments Porcine gelatin content analysisBoth the flowable and the MAP hemostatic agents were able to effectively control surgical bleeding in a novel severe liver injury model, however, the flowable gelatin–thrombin agent provided quicker and better bleed control.īleeding is a prevalent complication of liver surgery that negatively affects clinical outcomes. Kaplan–Meier survival analysis indicated similar rates of death between study groups (Logrank test p = 0.3395). The mean arterial pressure gradually recovered in the flowable group by 24 h, whereas in the MAP group, the mean arterial pressure was consistently stayed below baseline values. The rate of blood loss was significantly lower in the flowable group compared with the MAP group as measured from time of injury to minutes 3, 9, 12, and 120 (except for 6 min). The main outcome measure was total blood loss volume. We used a novel severe liver injury model that caused exsanguinating hemorrhage. Twenty swine were prospectively randomized to receive either active Flowable (Floseal) or passive MAP powder (PerClot) hemostatic agents. We have hypothesized that a single hemostatic agent might be as effective as a unique hemostatic surgical technique. Both surgical technique and hemostatic agents can be used together as a means of controlling bleeding. This study compares the effectiveness of two different types of hemostatic agents, one is an active flowable hemostat and the other is a passive hemostat made of modified absorbable polymers. High bloom strength bovine gelatin inhibited amplification of DNA from all three species.There are many surgical techniques (packing, Pringle maneuver, etc.) and hemostatic agents to manage hepatic bleeding in trauma surgery. Efficient tomato DNA amplification occurred without low bloom strength bovine gelatin in the reaction mixture. Relative to gelatin-free reactions, addition of low bloom strength bovine derived gelatin improved potato and blueberry DNA amplification, but was dependent on the primer used. Porcine derived gelatins inhibited the PCR or reduced reaction specificity, resulting in poorly resolved major bands and a smear of less abundant products on agarose gels. Furthermore, we show that substitution of bovine serum albumin (BSA) for gelatin increased DNA amplification yields and was required for optimizing the reaction conditions. Technical Abstract: Using a protocol for randomly amplified polymorphic DNA (RAPD) analysis which employs stringent annealing temperatures and relies on a polymerase chain reaction (PCR) buffer containing 1% Triton X-100 and 0.1% gelatin, we have demonstrated using tomato, potato, and blueberry DNA that the addition of the proper type of gelatin to the reaction mixture is important to obtain reliable amplification. Results of this research will assist researchers active in the construction of genetic linkage maps, tagging of desirable genes, fingerprinting cultivars, and conducting population and phylogenetic studies. Furthermore, we show that substitution of bovine serum albumin (BSA) for gelatin increased DNA amplification yields and was superior to gelatin for optimizing reaction conditions. Gelatin derived from porcine skin of high or low gelling strength inhibited the PCR whereas addition of low gelling strength gelatin derived from bovine skin had a positive effect on DNA amplification. Gelatin or bovine serum albumin (BSA) and nonionic detergents are often included in PCR reactions to help stabilize the Taq polymerase enzyme, a key component of the reaction. Using a protocol for RAPD analysis which employs stringent DNA annealing conditions and high levels of detergent and gelatin, we have demonstrated that addition of the proper type of gelatin to the reaction mixture is important to obtain reliable DNA amplification. One of these techniques generates a type of molecular marker termed randomly amplified polymorphic DNA (RAPD). Interpretive Summary: Polymerase chain reaction (PCR) has revolutionized many standard molecular biological techniques.
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